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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Generation of multilineage liver organoids with luminal vasculature and bile ducts from human pluripotent stem cells via modulation of Notch signaling

Fig. 3

Optimization of Notch signaling activation for generating mLOs. A Schematic timeline of the differentiation of mLOs for 16 days in DAPT-containing DM or DM lacking DAPT [DM(-DAPT)]. Days of DAPT removal are indicated in parentheses from day 1 (-DAPT/D1) to 10 (-DAPT/D10) of differentiation. B Phase-contrast and immunofluorescent images of E-cadherin (hepatic epithelium) and Jagged 1 (Notch ligand) in mLOs after treatment with DAPT at different time points of differentiation. The insets show high-magnification images of E-cadherin staining. Scale bar, 200 μm. C Quantification of immunoreactive signals for E-cadherin and Jagged 1 in B. Each black dot represents the percent positive area of the immunoreactive signal in each mLO. **p < 0.01, ***p < 0.001, and ****p < 0.0001 compared to DM by unpaired t tests

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