Fig. 6

Immunofluorescent staining for endogenous neurogenesis in SVZ and DG. a, b Immunofluorescent staining for BrdU, Dcx, and DAPI shows the endogenous neurogenesis in SVZ and DG. SVZ: Scale bar = 100 μm. DG: Scale bar = 200 μm. c High-magnification photographs of a representative immunofluorescent staining for BrdU/Dcx double-positive cells in the SVZ (yellow arrows). Scale bar = 100 μm. d, e ANOVA detected significant treatment effects on endogenous neurogenesis with significantly increased numbers of BrdU/Dcx double-positive cells in SVZ and DG in the SB623 + Ex group compared to the vehicle, Ex, and SB623 groups, respectively (each group: n = 5, SVZ: F_{(3, 16)} = 18.9, p < 0.001; F_{(3, 16)} = 13.7, DG: p < 0.001). The Ex group and the SB623 group had significantly increased numbers of BrdU/Dcx double-positive cells in SVZ and DG compared to the vehicle group (SVZ: mean ± SE, ^*p < 0.05, ^**p < 0.01 vs. vehicle group, ^#p < 0.05 vs. SB623 + Ex group; DG: ^*p < 0.05, ^**p < 0.01 vs. vehicle group, ^#p < 0.05 vs. SB623 + Ex group). Abbreviation: SVZ: subventricular zone; DG: dentate gyrus; BrdU: 5-bromo2′-deoxyuridine, Dcx: Doublecortin, DAPI: 4′ 6-Diamidino-2-phenylindole