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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Combining single-cell transcriptomics and CellTagging to identify differentiation trajectories of human adipose-derived mesenchymal stem cells

Fig. 6

(A) Western blot analysis shows that the expression of SERPINE2 was upregulated after its overexpression, full-length blots are presented in Additional file 1: Fig. S1 (B, C, D, E) Activation of SERPINE2 promotes osteogenic differentiation of hADSCs, as observed under the microscope. Alizarin Red staining shows that the cell mineralization ability was enhanced after activating SERPINE2 compared with the control group. ALP staining shows an increase in ALP content after overexpression of SERPINE2 compared to the control group. (F) qRT-PCR was used to detect the expression levels of the osteogenesis-associated genes ALP, BGLAP, and Runx2 during the osteoblast differentiation of hADSCs

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