Fig. 6

Histologic and immunohistochemical assessments of diabetic osseous defect healing at days 7 and 28. A Representative images of hematoxylin and eosin staining. Magnification: 40 × . Scale bar: 1 mm. B Representative images of immunohistochemical staining for bone sialoprotein (BSP). Magnification: 100 × . Scale bar: 100 µm. C The ratio of proliferating cells (PCNA( +) cells) in the osseous defect at days 7 and 28. D Histomorphometric analysis of the defect fill and new bone (NB) bridging at day 28. Dashed lines indicate the borders of osseous defects. There is no downstream processing or averaging to adjust the resolution of the microscopic images. (Among groups with diabetes, significant difference compared to DB-CL: ^*p < 0.05, ^**p < 0.01, ^***p < 0.001)