Fig. 8

BMSCs-FoxM1 coculture promotes the proliferation, migration, and tube formation ability of ECs. A EdU was measured for EC proliferative capacity, of which the blue color indicated the nuclear localization and the red color indicated the proliferation-active cells (magnification 100×) B Quantitative analysis was conducted by calculating the percentage of proliferation-active cells, and the results suggested that BMSCs-FoxM1 coculture dramatically promoted EC proliferation capacity after LPS-induced injury compared to BMSCs-Vector coculture groups. C The scratch assay was conducted to assess the migration capability of EC, and representative images of the scratches at different time points at 0 h and 24 h are shown (magnification 100×). D Quantitative analysis of the changes in the scratched areas was performed using Image J software, and results suggested that the migration ability of ECs was significantly increased in the BMSCs-FoxM1 coculture group compared to BMSCs-Vector coculture group. E Tube formation assay was performed to detect EC angiogenic capacity, and Calcein AM fluorescent dye was used to enhance the visibility of tube and network formation in Matrigel (magnification 100× , 200×), along with the trajectories of tubes and networks were also depicted accordingly. F Quantitative analysis suggested that coculture with BMSCs-FoxM1 significantly promoted the EC tube formation ability compared to that in BMSCs-Vector coculture group. Values are expressed as mean ± SEM. (n = 3, *Compared with control group, *p < 0.05, **p < 0.01, ***p < 0.001; #compared with LPS group, #p < 0.05, ##p < 0.01, ###p < 0.001)