Table 1 Primer sequences used for qRT-PCR
Gene | Forward (5′-3′) | Reverse (5′-3′) |
|---|---|---|
GADPH | CATCACTGCCACCCAGAAGACTG | ATGCCAGTGAGCTTCCCGTTCAG |
TGF-β | CTGCTGACCCCCACTGATAC | AGCCCTGTATTCCGTCTCCT |
Hif-1α | CCTGCACTGAATCAAGAGGTTGC | CCATCAGAAGGACTTGCTGGCT |
Interleukin-1β | TGGACCTTCCAGGATGAGGACA | GTTCATCTCGGAGCCTGTAGTG |
Interleukin-6 | TGGTTCCTACCCCAATTTCCA | GTCTTGGTCCTTAGCCACTCC |
Collagen type 1α1 | CCTCAGGGTATTGCTGGACAAC | CAGAAGGACCTTGTTTGCCAGG |
Collagen type 3α1 | GACCAAAAGGTGATGCTGGACAG | CAAGACCTCGTGCTCCAGTTAG |
Fibronectin | CCCTATCTCTGATACCGTTGTCC | TGCCGCAACTACTGTGATTCGG |