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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Endothelial colony forming cell administration promotes neurovascular unit development in growth restricted and appropriately grown fetal lambs

Fig. 4

A-D Representative photomicrographs of double-label laminin (green) and GFAP (red) immunofluorescence staining in the sCWM. E Data are mean ± SEM average M2 Manders’ co-localisation coefficient (MCC) per field of view as calculated in the Cx, PVWM, sCWM, and SVZ from AGA (Black; n = 7), FGR (Grey; n = 5), AGA + ECFC (Black patterned; n = 6), and FGR + ECFC (Grey patterned; n = 6). Data were examined using a two-step analysis, with AGA and FGR compared first via unpaired t test, and then, all groups were compared using two-way ANOVA with FGR and ECFC treatment as variables. Two-way ANOVA results for main effects (PECFC, PFGR) and interaction (PINT) for each brain region are presented below the relevant graph (NS = P > 0.05). #P < 0.05 for Kolmogorov–Smirnov test of residuals in two-way ANOVA analysis of PVWM

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