Fig. 1
Proteomic analysis of H19 downstream targets and ECM-related processes. a H19 expression after transfection of MSCs, isolated from osteoporotic patients, with two alternative small interference RNAs against H19 (siH19-1 and siH19-2) or the respective control (siCTR), cultured for 3 days in osteogenic-inducing conditions (N = 6). b H19 subcellular location in U2OS osteosarcoma cells. Levels were normalized against the whole-cell content. XIST and MALAT1, two nuclear lncRNAs, were used as quality controls. c Volcano plot of the proteins identified after transfection with siH19 versus control (FC ≥ |1.25|) and p-value < 0.05. The y-axis corresponds to the − Log10(p-value) of a certain protein and the x-axis the correspondent Log10(FC). d Gene ontology (GO) enrichment analysis, according to PANTHER classification system. The x-axis shows the −Log10(p-value) associated with determined GO term (y-axis). e Venn diagram showing the extracellular proteins differentially expressed by H19 knockdown. f Proteins associated with the GO terms “non-structural extracellular” and “extracellular matrix” with p < 0.01 and a minimum of four unique peptides (left) and with the GO term “extracellular structural activity” (right) and respective heatmap.