Skip to main content
Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Long non-coding RNA H19 regulates matrisome signature and impacts cell behavior on MSC-engineered extracellular matrices

Fig. 2

Effect of H19 knockdown on osteogenic differentiation, ECM composition, proliferation and apoptosis in MSCs. a mRNA expression levels of FBN1, VNT and CTHRC1 after H19 knockdown compared with control (N = 6). b Representative image and quantification of FBN1, VTN and CTHRC1 protein levels 10 days after culture in osteogenic-inducing conditions (N = 6; scale: 50 μm). c COL1 staining (left) and quantification (right) at day 7 of differentiation (COL1: red; N = 6; scale: 100 μm). d ECM components (COL1A1, COL1A2 and COL3A1) and e Osteogenic markers (ALP and RUNX2) expression levels at day 3 after transfection and incubation in osteogenic-inducing conditions (N = 6). f ALP (N = 6) and g Alizarin (N = 6) staining after transfection and culture for 7 and 14 days, respectively, in osteogenic-inducing conditions. h Representative fluorescence profile (resorufin) measured every 24 h for 7 days in transfected MSCs; quantification of the percentage of cells in proliferation (ki-67+/DAPI+) 3 days after siH19 or siCTR transfection; and representative images of MSC stained for the proliferation marker Ki-67 protein (red) and nuclear DNA labeled with DAPI (blue) (N = 6; scale: 50 μm) (RFU: relative fluorescence units). i Flow cytometry analysis of Annexin V/PI staining and quantification of viable (Annexin V PI), early apoptotic (Annexin V+ PI), or late apoptosis and dead cells (Annexin V PI+ or Annexin V+ PI+) (N = 6). siCTR: MSC transfected with scrambled control sequence; siH19: MSC transfected with small interference RNA against H19.

Back to article page