Fig. 5

HFSCs and HFSCs/HGF promoted angiogenesis and protected blood–brain barrier. A–D Immunofluorescent staining of vWF indicating microvessels in the penumbra. Scale bar = 50 µm. E and F The quantitative analysis of the density and length of microvessels. G The percentage of water content in all groups. H Western blot analysis of the tight junction proteins in the ischemic hemispheres. I and J The quantitative analysis of the ratios of ZO-1 and occludin to β-actin, respectively. Values are the mean ± SD. *P < 0.05 Saline group vs. Control group, ^#P < 0.05 HFSCs group vs. Saline group, ^∆P < 0.05 HFSCs/HGF group vs. HFSCs/Vehicle group, n = 6