Fig. 1From: Dose-specific efficacy of adipose-derived mesenchymal stem cells in septic miceDose-related effects of ADSCs on CLP-induced mortality and multi-organ damage. Six hours after sham or CLP surgery, mice were intravenously infused with vehicle solution or different doses of ADSCs, including low-dose (1 × 107 cells/kg, ADSCs1), medium-dose (2 × 107 cells/kg, ADSCs2), and high-dose (4 × 107 cells/kg, ADSCs4). The survival rates were monitored every 12 h for 168 h. n = 4–45 (A). Changes in multi-organ function after ADSCs treatment in septic mice were determined by examining the plasma levels of ALT, AST, BUN, and LDH at 6 h after ADSCs treatment (12 h after CLP). n = 5 in each group (B). H&E staining was performed to assess the severity of CLP-induced structural damage in multiple organs, including the lung, liver, and kidney, at 6 h after ADSCs treatment. Images were taken by Nikon 80i microscopy and SPOT Software at a resolution of 96 dpi, and processed in Adobe Photoshop at a resolution of 600 dpi. No downstream processing was utilized. Scar bar = 200 μm (C). Lung: abnormal alveolar collapse and alveolar septal inflammation (dotted line) and arteriole thrombosis (black arrow); Liver: abnormal hyperplasia of bile duct epithelium (black arrow) and hepatocyte disarrangement and hydropic degeneration (dotted line); Kidney: abnormal acute tubular necrosis (blue triangle, inflammatory infiltration (black dotted arrow) and glomerular damage (black arrow)). Pathology scoring was blindly assessed to indicate the degree of organ damage (D). n = 5 in each group. *, p < 0.05, significantly different from the CLP + Vehicle group; #, p < 0.05, significantly different from the CLP + ADSCs4 groupBack to article page