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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Flow-dependent shear stress affects the biological properties of pericyte-like cells isolated from human dental pulp

Fig. 3

Endothelial differentiation of hDPSCs. A Morphological analysis of undifferentiated and endothelial differentiated hDPSCs after 3, 5 and 7 days of induction. Red dashed square and rectangle highlight the cord-like vessel morphology of differentiated hDPSCs. Scale bar: 50 μm. B Phalloidin staining shows the morphological rearrangement of hDPSCs toward endothelial lineage. Scale bar: 10 μm. C–D Western blot and immunofluorescence analyses of PDGFR-β in hDPSCs after endothelial differentiation. hDPSCs undiff and HUVEC were used as controls. E Western blot analysis of cleaved caspase 3 in hDPSCs following endothelial differentiation. Full-length blots are presented in Additional file 1: Figure S1A. Densitometry analysis is shown in histograms. Data are represented as mean ± SD, and statistical analysis on C and E was performed by one-way ANOVA followed by Dunnett post hoc test; ***P < 0.001, **P < 0.01 vs hDPSCs undiff (n = 3) (C), ***P < 0.001 positive ctrl vs hDPSCs (n = 3) (E)

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