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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Flow-dependent shear stress affects the biological properties of pericyte-like cells isolated from human dental pulp

Fig. 4

Evaluation of angiogenic potential of hDPSCs. A Western blot analysis of eNOS, VEGF, ANGPT1 and Tie2 in endothelial differentiated hDPSCs. hDPSCs undiff and HUVEC were used as controls. Full-length blots are reported in Additional file 1: Figure S1B. B The expression of Tie2 and VEGF is shown by immunofluorescence images. Nuclei were counterstained with DAPI. Scale bar: 10 μm. C Tube formation assay showing tube-like structures in undifferentiated and endothelial differentiated hDPSCs. Histograms reporting the mean \(\pm\) SD number of tube-like structures in 15 × 103 μm2. Scale bar: 50 μm. Data are represented as mean ± SD, and statistical analysis on A was performed by one-way ANOVA followed by Dunnett post hoc test; ***P < 0.001, **P < 0.01, *P < 0.05 vs hDPSCs undiff (n = 3). Statistical analysis on C was carried out by unpaired Student’s t test; ***P < 0.001 vs hDPSCs undiff (n = 3)

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