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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Flow-dependent shear stress affects the biological properties of pericyte-like cells isolated from human dental pulp

Fig. 6

Mechanotransduction induced in hDPSCs by FSS. A Heatmap of RPPA analysis carried out on phosphorylated proteins in hDPSCs, after static and dynamic conditions. Histograms showing the most prominent signaling pathways. Data are represented as mean ± SD, and statistical analysis was carried out by unpaired Student’s t test; ***P < 0.001, **P < 0.01 vs hDPSCs in static condition (n = 5). B Immunofluorescence analysis carried out on hDPSCs, under static and dynamic conditions, shows the expression of endothelial markers VEGF, ANGPT1 and eNOS. Pseudocolor analysis of each endothelial marker is reported on the right side. Nuclei were counterstained by DAPI. Scale bar: 10 μm. C Tube formation assay showing tube-like structures in endothelial differentiated hDPSCs under static and dynamic conditions. Histograms reporting the mean \(\pm\) SD number of tube-like structures in 15 × 103 μm2. Scale bar: 50 μm. Data are represented as mean ± SD and statistical analysis was carried out by unpaired Student’s t test; ***P < 0.001 vs hDPSCs diff static condition (n = 5)

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