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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Single cell and lineage tracing studies reveal the impact of CD34+ cells on myocardial fibrosis during heart failure

Fig. 1

Single-cell RNA sequencing (scRNA-seq) analysis of cardiac hypertrophy in a mouse model of pressure overload. A Schematic depicting the pipeline of transverse aortic constriction (TAC) surgery and tissue harvesting of Cd34-CreERT2; Rosa26-tdTomato mouse for scRNA-seq. Heart tissue was harvested 0, 4 and 12 weeks after TAC surgery. Single cells were then isolated from the left ventricle and subjected to scRNA-seq. N = 6 per group. B Representative echocardiography of mice at different stages of pathological cardiac hypertrophy; H&E staining of heart at 0, 4 and 12 weeks after TAC surgery. C Echocardiographic measurements of left ventricle ejection fraction (EF) and fractional shortening (FS) in mice from TAC 0 or different TAC groups. TAC 0 group, n = 19; TAC 4 group, n = 23; TAC 12 group, n = 16. Data represent mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001. D Umap plot displaying the major cell types and color-coded cell clusters at different stages of pathological cardiac hypertrophy. n = 59,505 cells. E Dot plot showing expression levels of top five differentially expressed genes in each cell cluster. Dot size reflects the percentage of cells expressing the selected gene in each cell cluster

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