Fig. 2

Characterization of heart composition at different stages of pathological cardiac hypertrophy by ScRNA-seq. A Umap plot displaying the major cell types and color-coded cell clusters of CD34-CreERT2; Rosa26-tdTomato mouse at 0, 4 and 12 weeks after TAC surgery. n = 21,881 cells. B Bar chart showing the percentage of major cell types among different datasets at different stages of pathological cardiac hypertrophy. C. Umap plot displaying distribution of fibroblast subpopulations among different datasets at 0, 4 and 12 weeks after TAC surgery. n = 15,822 cells. resolution = 0.5. D Violin plot showing the expression of selected marker gene of each subclusters. E Bar chart showing the percentage of subclusters in datasets at 0, 4 and 12 weeks after TAC surgery. F Dot plot showing the expression of fibroblast genes. Dot size reflects the percentage of cells expressing the selected gene in each cell cluster. G Violin plot showing the GO datasets score among fibroblast subclusters. H Volcano plot showing the DEGs between datasets (p-value < 0.01 and log2FC > 1 was labeled). I Go analysis revealed biological processes enriched with DEGs between different groups (0, 4 and 12 weeks after TAC surgery) (DEGs log2FC > 0.5 and pct.1/pct.2 > 1 were used). T indicates TAC