Fig. 3

Characterization of CD34 cell-derived cells at different stages of pathological cardiac hypertrophy by ScRNA-seq. A Umap plot displaying the major cell types and color-coded CD34 derived cell clusters of Cd34-CreERT2; Rosa26-tdTomato mouse at 0, 4 and 12 weeks after TAC surgery. n = 31,025 cells. B Umap plot displaying distribution of fibroblast subpopulations among different datasets at 0, 4 and 12 weeks after TAC surgery. n = 27,626 cells. resolution = 0.35. C Violin plot showing the expression of selected marker gene of each subcluster. D Heatmap showing cluster similarity of fibroblast subclusters in total and CD34 + cell-derived datasets. E Bar chart showing the percentage of subclusters in datasets at different stages of pathological cardiac hypertrophy. F Bar plot showing the GO enrichment of selected subclusters. G Representative images showing specific cell identification by staining with tdTomato, Vimentin, DDR2, PDGFRA (Scale bar = 20 μm and 100 μm). n = 8 per group. H Graph showing percentage of tdTomato expression in vimentin + , DDR2 + , PDGFRA + fibroblast. Data represent mean ± SEM, n = 8. *P < 0.05; **P < 0.01; ***P < 0.001. Tdm, tdTomato