Fig. 4

Characterization of non-bone marrow CD34 + cell-derived cells at 4 weeks of pathological cardiac hypertrophy by ScRNA-seq. A Umap plot displaying the major cell types and color-coded non-bone-marrow-derived CD34 + cell-derived cells at 4 weeks after TAC surgery. BMT TAC 4R, transplanting bone marrow cells from wild-type C57BL/6 J mice to irradiated CD34-CreERT2;Rosa26-tdTomato, further subjected to TAC surgery; TAC 4 R, normal CD34-CreERT2;Rosa26-tdTomato mice and subjected to TAC surgery. n = 14,534 cells. B Umap plot displaying distribution of fibroblast subpopulations in non-bone marrow CD34 + cell-derived cells at 4 weeks after TAC surgery. n = 10,039 cells. resolution = 0.2. C Heatmap showing the expression of top ten differentially expressed genes in each cell subclusters. D Bar chart showing the percentage of subclusters in datasets between T4 R and BMT T4R group. E Violin plot showing the expression of selected marker gene of each subclusters. F Dot plot showing the expression of genes related to ECM proteins and fibroblast activation between T4 R and BMT T4R group. G Immunofluorescence staining of tdTomato and fibroblast markers (vimentin and DDR2) in bone marrow transplantation of WT (wild-type C57BL/6 J) to CD34-CreERT2;Rosa26-tdTomato mice. n = 7 per group. H Immunofluorescence staining of tdTomato and fibroblast markers (vimentin, DDR2 and PDGFRA) in bone marrow transplantation of CD34-CreERT2; Rosa26-tdTomato mice to WT (wild-type C57BL/6 J) (Scale bar = 20 μm and 100 μm). n = 7 per group. BMT, bone marrow transplantation; T4, TAC 4