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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Choosing the source of healthy controls for studies on myeloid malignancies: all bone marrow cells are created equal, but some are more equal than others

Fig. 2

Analysis of the functional properties of MSC cultured from iliac crest aspirations and femoral heads. A The cumulative population doubling (PD) of MSC is presented according to the number of days since the beginning of cell culture (mean). B Representative pictures of β-galactosidase staining of MSC at passage P6 from samples with low level of senescence (top) or high level of senescence (bottom), scale bar = 20 µm. C Quantification of the percentage of senescent cells at passage P6 (mean ± SD, ns = non significant using a Mann–Whitney test). D Representative pictures of adipogenic (top) and osteogenic (bottom) differentiation capacity at passage P3, scale bar = 50 µm. E, F Quantification of adipogenic and osteogenic differentiation capacity (mean ± SD, arbitrary units). G, H Proportions of HSPC subpopulation (G) and number of cells (H) after 4 days of co-culture of HSPC and MSC from the iliac crest or femoral head (mean percentage ± SD)

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