Fig. 1

MiR-21 improves viability and proliferative activity in BMSCs isolated from senile osteoporotic SAM/P6 mice. A The representative photographs (z-stack) of cells surface markers (CD44, CD45, CD73, CD90 and CD105) in BMSCs isolated from BALB/c and SAM/P6 mice. The staining intensity of visualised antigens was presented as bar graph (B). The level of miR-21-5p was analysed in bone tissue samples delivered from BALB/c and SAM/P6 mice (C), as well as in cell cultures after transfection with miR-21 mimic (D). E The representative photographs (z-stacks) of cells stained with Hoechst 33342 and Ki67 protein in BMSCs isolated from BALB/c and SAM/P6 mice. The staining intensity of visualised F nuclei and G Ki67 protein were presented as bar graphs. The RT-qPCR technique was used to present the gene expression of H Bax, I Bcl-2 and J Bax/Bcl-2 ratio. The RT-qPCR measurements were performed using RQMAX method and presented in a log scale. The confocal microscope photographs were taken under 630-fold & 1000-fold magnification and the scale bars are equal to 40 µm and 10 µm, respectively. Significant differences between groups are indicated with asterisk: *p < 0.05, **p < 0.01, *** and p < 0.001. Non-significant differences are marked as ns