Fig. 2

MiR-21 reduces autophagy and regulates mitochondria dynamics in BMSCs isolated from senile osteoporotic SAM/P6 mice. A The representative photographs (z-stacks) of cells’ nuclei stained with lysosomes, LAMP2 and mTOR protein in BMSCs isolated from BALB/c and SAM/P6 mice. The staining intensity of visualised B lysosomes, C LAMP2 protein and D mTOR protein were presented as bar graphs. The RT-qPCR technique was used to present the gene expression of E Ppar-γ, F mTOR and G Lamp2. H The representative photographs (z-stacks) of nuclei with stained MFN-1 protein and mitochondrial network in BMSCs isolated from BALB/c and SAM/P6 mice. The images were supplemented with MicroP visualisation of mitochondria morphology and presented as a bar graph (I). The staining intensity of visualised J MFN-1 protein and K mitochondria network were presented as bar graphs. The RT-qPCR technique was used to present the gene expression of L Mfn-1 and M Mff. The RT-qPCR measurements were performed using RQMAX method and presented in a log scale. The confocal microscope photographs were taken under 630-fold and 1000-fold magnification and the scale bar is equal to 40 µm. Significant differences between groups are indicated with asterisk: *p < 0.05, **p < 0.01, ***p < 0.001. Non-significant differences are marked as ns