Fig. 4

MiR-21 inhibits osteoclastogenesis in BMSCs isolated from senile osteoporotic SAM/P6 mice. A The representative images (z-stacks) of cells’ nuclei, actin cytoskeleton and TRAP protein in BMSCs isolated from BALB/c and SAM/P6 mice. The confocal microscope photographs were taken under 630-fold magnification and the scale bar is equal to 40 µm. The staining intensity of TRAP protein was presented as a bar graph (B). The RT-qPCR technique was used to present the gene expression of C Trap, D Ctsk, E Mmp-9, F Nfatc-1 and G Pu.1. The RT-qPCR measurements were performed using RQMAX method and presented in a log scale. Significant differences between groups are indicated with asterisk: *p < 0.05, **p < 0.01, ***p < 0.001. Non-significant differences are marked as ns