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Table 1 The characteristic of primers used in RT-qPCR

From: MiR-21-5p regulates the dynamic of mitochondria network and rejuvenates the senile phenotype of bone marrow stromal cells (BMSCs) isolated from osteoporotic SAM/P6 mice

Gene

Primer Sequence 5′-3′

Annealing [°C]

Accession No

Ppar-γ

F:CTCTGCTGGGGATCTGAAGG

58.8

NM_001308354.1

R:GGAATGCGAGTGGTCTTCCA

mTOR

F:CTTGGAGAACCAGCCCATAA

60.0

NM_020009.2

R:CTGGTTTCACCAAACCGTCT

Lamp2

F:CTTAGCTTCTGGGATGCCCC

60.0

NM_001017959.2

R:TCATCCAGCGAACACTCCTG

Mfn-1

F:ATCACTGCAATCTTCGGCCA

60.0

NM_024200.4

R:AGCAGTTGGTTGTGTGACCA

Mff

F:TCACATTTGGTGAGTGGGGC

60.0

NM_001372412.1

R:TTTTCCGGGACCCTCATTCG

Bcl-2

F:ATCGCCCTGTGGATGACTGAG

58.8

NM_000633.2

R:CAGCCAGGAGAAATCAAACAGAGG

Bax

F:ACCAAGAAGCTGAGCGAGTGTC

58.8

NM_001291428.1

R:ACAAAGATGGTCACGGTCTGCC

Mmp-9

F:GATGCCAACCTCCTCAACGA

60.0

NM_053056.2

R:GGAAGCGGTCCAGGTAGTTC

Runx-2

F:TCCGAAATGCCTCTGCTGTT

58.8

NM_001271630.1

R:GCCACTTGGGGAGGATTTGT

Coll-1

F:CAGGGTATTGCTGGACAACGTG

61.4

NM_007742.4

R:GGACCTTGTTTGCCAGGTTCA

Opn

F:AGACCATGCAGAGAGCGAG

57.3

NM_001204203.1

R:GCCCTTTCCGTTGTTGTCCT

Ocl

F:GGTGCAGACCTAGCAGACACCA

57.0

NM_001032298.3

R:CGCTGGGCTTGGCATCTGTAA

Opg

F:AGCCACGCAAAAGTGTGGAA

58.8

NM_008764.3

R:TCCTCTCTACACTCTCGGCA

Alpl

F:TTCATAAGCAGGCGGGGGAG

60.0

NM_007431.3

R:TGAGATTCGTCCCTCGCTGG

Bmp-2

F:CTACAGGGAGAACACCCGGA

60.0

NM_007553.3

R:GGGGAAGCAGCAACACTAGAA

Trap

F:GTCTCTGGGGGACAATTTCTACT

60.0

XM_006509945.3

R:GTTTGTACGTGGAATTTTGAAGC

Ctsk

F:TAACAGCAAGGTGGATGAAATCT

60.0

NM_011613.3

R:CTGTAGGATCGAGAGGGAGGTAT

Nfatc-1

F:TTCGAGTTCGATCAGAGCGG

60.0

NM_001164112.1

R:AGGTGACACTAGGGGACACA

Pu.1

F:GAGAAGCTGATGGCTTGGAG

60.0

NM_001378899.1

R:TTGTGCTTGGACGAGAACTG

Gapdh

F:GTCAGTGGTGGACCTGACCT

58.8

NM_001289746.1

R:CACCACCCTGTTGCTGTAGC

B2m

F:CATACGCCTGCAGAGTTAAGCA

58.8

NM_009735.3

R:GATCACATGTCTCGATCCCAGTAG

miR-7a-5p

TGGAAGACTAGTGATTTTGTTGT

58.8

MIMAT0000677

miR-17-5p

CAAAGTGCTTACAGTGCAGGTAG

58.8

MIMAT0000649

miR-21a-5p

TAGCTTATCAGACTGATGTTGA

58.8

MIMAT0000530

miR-124-3p

TAAGGCACGCGGTGAATGCC

58.8

MIMAT0000134

miR-145-5p

GTCCAGTTTTCCCAGGAATCCCT

58.8

MIMAT0000437

miR-203a-3p

GTGAAATGTTTAGGACCACTAG

58.8

MI0000283

miR-223-3p

TGTCAGTTTGTCAAATACCCCA

58.8

MIMAT0000280