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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: BM-MSCs overexpressing the Numb enhance the therapeutic effect on cholestatic liver fibrosis by inhibiting the ductular reaction

Fig. 3

BM-MSCsNumb-OE transplantation inhibits the activation of the Notch signaling pathway in the liver. a Numb, RBP-Jκ and Hes1 immunostaining (× 400). b Protein and mRNA expression levels of Numb, RBP-Jκ, and Hes1 (n = 6/per group) (Full-length blots are presented in Additional file 2: Figs. 3–5); c CK7 and CK19 immunostaining (× 200). d CK7 and CK19 immunoblotting bands, gray-level integration and mRNA expression (n = 6/per group) (Full-length blots are presented in Additional file 2: Figs. 6 and 7). e CK7/EGFP (labeled BM-MSCOE-EV and BM-MSCNumb-OE, the same below) immunofluorescence costaining (× 200). f CK19/EGFP immunofluorescence costaining (× 200). g Alb/EGFP immunofluorescence costaining (× 200), and the costaining area ratio of Alb/EGFP. h HNF4α/EGFP immunofluorescence costaining (× 200), and the costaining cells ratio of HNF4α/EGFP. i EGFP/CD90/Alb immunofluorescence costaining (× 400). *P < 0.05; **P < 0.01

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Stem Cell Research & Therapy

ISSN: 1757-6512