Table 1 Primer pairs and probes used for real-time PCR
Gene |  | Primer sequence (5′ → 3′) | Note |
|---|---|---|---|
α-SMA | Forward | AAT GGC TCT GGG CTC TGT AA | SYBR Green |
Reverse | TCT CTT GCT CTG GGC TTC AT | ||
Col (1) | Forward | ACG TCC TGG TGA AGT TGG TC | SYBR Green |
Reverse | TCC AGC AAT ACC CTG AGG TC | ||
Col (4) | Forward | TTT CCA GGG TTA CAA GGT GT | SYBR Green |
Reverse | AGT CCA GGT TCT CCA GCA TC | ||
TGF-β1 | Forward | ATT CCT GGC GTT ACC TTG G | SYBR Green |
Reverse | AGC CCT GTA TTC CGT CTC CT | ||
TNF-α | Forward | GAC GTG GAA CTG GCA GAA GAG | SYBR Green |
Reverse | TTG GTG GTT TGT GAG TGT GAG | ||
CK7 | Forward | AGG AAC AGA AGT CAG CCA AGA G | SYBR Green |
Reverse | GCA ACA CAA ACT CAT TCT CAG C | ||
CK19 | Forward | GAT CTG CGT AGT GTG G-3′ | SYBR Green |
Reverse | AAA ACC AAA CTG GGG ATG-3′ | ||
Numb | Forward | GCT ACT TTC GAT GCC AGT AGA ACC A | SYBR Green |
Reverse | CTG TTG CCA GGA GCC ACT GA | ||
RBP-Jκ | Forward | TTG CTT ACC TTC AGG CGT GTG | SYBR Green |
Reverse | GCC CAA TGA GTC TGC TGC AA | ||
Hes1 | Forward | GAC GGC CAA TTT GCT TTC | SYBR Green |
Reverse | GAC ACT GCG TTA GGA CCC | ||
Notch1 | Forward | TGG ATG AGG AAG ACA AGC ATT A | SYBR Green |
Reverse | GAA AAG CCA CCG AGA TAG TCA G | ||
Notch2 | Forward | GAG GAA GAA GTG TCT CAA | SYBR Green |
Reverse | GTG GCA TCA GAA ACA TAT G | ||
Notch3 | Forward | GAC AAG GAC CAC TCC CAC TACT | SYBR Green |
Reverse | ATC CAC ATC ATC CTC ACA ACT G | ||
Notch4 | Forward | TGT CAG GAA CCA GTG TCA GAA C | SYBR Green |
Reverse | CCT GGG CTT CAC ATT CAT CTA T | ||
JAG1 | Forward | CCA TCA AGG ATT ATG AGA AC | SYBR Green |
Reverse | TGG TGC TTA TCC ATA TCA | ||
JAG2 | Forward | AAA TGA GTG GTC CGT GGC AGA | SYBR Green |
Reverse | TGG TTG GAA GCC TTG TCT GCT | ||
DLL1 | Forward | GTG TGC AGA TGG TCC TTG CTT C | SYBR Green |
Reverse | CTG ACA TCG GCA CAG GTA GGA G | ||
DLL3 | Forward | CTG AGG TTA CAA GAC GGT GCT | SYBR Green |
Reverse | GTA AAT GGA AGG GGC TGG TAT G | ||
DLL4 | Forward | GCA GAA CCA CAC ACT GGA CTA T | SYBR Green |
Reverse | TGG CAC CTT CTC TCC TAA ACT C | ||
Sox9 | Forward | GAA AGA CCA CCC CGA TTA CAA G | SYBR Green |
Reverse | AAG ATG GCG TTA GGA GAG ATG TG | ||
EpCam | Forward | TGT GGA CAT AGC TGA TGT GGC TTA C | SYBR Green |
Reverse | CAC CCT CAG GTC CAT GCT CTT A | ||
LNX1 | Forward | TGC TGC CAG GAG ACA TCA T | SYBR Green |
Reverse | CAT TGC TTC TGC TAC GGA ACT T | ||
LNX2 | Forward | ACA CAG ATT GAG GGT GAA ACT | SYBR Green |
Reverse | GGT CCA CAC AGG AAG AGG T | ||
ITCH | Forward | ATG GGA GAT TTG TCA GTT TGT C | SYBR Green |
Reverse | CAG CGT CAT TCT GTG TAG CA | ||
Alb | Forward | AAG GCA CCC CGA TTA CTC CG | SYBR Green |
Reverse | TGC GAA GTC ACC CAT CAC CG | ||
HNF4α | Forward | CGG GCC ACT GGC AAA CAC | SYBR Green |
Reverse | GTA ATC CTC CAG GCT CAC | ||
GAPDH | Forward | GGC ACA GTC AAG GCT GAG AAT G | SYBR Green |
Reverse | ATG GTG GTG AAG ACG CCA GTA |