Fig. 1

Characterization and biodistribution of PGE2-MSCs and MSCs. A Schematic representation of the double-fusion (DF) firefly luciferase (Fluc) and green fluorescent protein reporter gene containing Fluc and GFP driven by ubiquitin promotion. B Brightfield and fluorescence microscopy showing GFP expression in MSCs and PGE2-MSCs. C, D The quantification BLI of MSCs and PGE2-MSCs shows a robust correlation between cell number and Fluc activity. The signal activity was represented by photons/s/cm²/steradian. E, F Fluc signals were tracked by BLI analysis to monitor the retention of transplanted PGE2-MSCs and MSCs in vivo. The signal activity was represented by photons/s/cm²/steradian