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Fig. 7 | Stem Cell Research & Therapy

Fig. 7

From: Inflammatory stimulation of astrocytes affects the expression of miRNA-22-3p within NSCs-EVs regulating remyelination by targeting KDM3A

Fig. 7

miRNA-22-3p had a biological effect similar to that of NSCs-EVs in regulating the differentiation of NSCs in vitro and promoting axonal regrowth and remyelination in vivo. A, B The PCR results showed that the expression of miRNA-22-3p in NSCs-EVs was increased after coculture with LPS-AS-CM or BMP2 for 24 h (A) and was inhibited by the addition of Noggin in the presence of LPS-AS-CM or BMP2 (B) (n = 3, data are the mean ± S. D., ∗p < 0.05). C The effects of the miRNA-22-3p mimics and inhibitors were confirmed by PCR after 24 h of transfection (n = 3, data are the mean ± S. D., ∗p < 0.05). D, E The transfection of miRNA-22-3p promoted the differentiation of NSCs into oligodendrocytes (n = 5; data are the mean ± S.; *p < 0.05). F The effect of miRNA-22-3p agomir in SCI rats was confirmed by PCR after continuous injection for 3 days (n = 3, data are the mean ± S. D., ∗p < 0.05). G,H,I The injection of miRNA-22-3p agomir increased the expression of Cnpase and MBP in the astrocytic scar and the percentage of Cnpase- and TUJ1- double-positive areas in lesion sites at week 4 post-SCI (n = 5; data are the mean ± S.D.; *p < 0.05). J The western blot results showed that the expression of Cnpase 4 weeks after SCI was increased by the injection of miRNA-22-3p agomir (n = 3, data are the mean ± S. D., ∗p < 0.05). K HE staining results showed that the percentage of the injured area was reduced by the injection of miRNA-22 agomir (n = 5, data are the mean ± S.D.; ∗p < 0.05, ns p > 0.05). L, M miRNA-22 agomir promoted the recovery of neurological function following SCI (n = 10, data are the mean ± S.D.; ∗p < 0.05, ns p > 0.05)

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