Fig. 1

Hypoimmunogenic iPSC characterization. A Flow cytometry analysis. Note the absence of surface expression of HLA-A, HLA-B, and HLA-C molecules in hi-iPSCs (red) following stimulation with IFN-γ when compared to wild-type iPSCs (wt-iPSCs, blue). Mean fluorescent intensity values are shown in (B). Bars represent means ± standard deviation from three independent experiments. *p < 0.05, **p < 0.01. C, D Expression of pluripotency markers. C Quantification of NANOG, POU5F1 (OCT4), and SOX-2 mRNA levels by RT-qPCR analysis. Data show the average and standard deviation of fold change values from three independent experiments (ns: non-significant). D Representative immunocytochemistry photomicrographs evidencing Nanog, Oct4, and SOX2. Bars 20 μm. E Representative phase-contrast photomicrographs of iPSC cultures, evidencing unaltered morphology and colony formation capacity in hi-iPSCs when compared to wt-iPSCs. “Colony formation” bars 300 μm, “Morphology” bars 60 μm. F Differentiation assay into ectoderm germ layer. Representative immunocytochemistry photomicrographs of cells differentiated from both wt-iPSCs and hi-iPSCs, expressing the ectoderm markers Nestin and Beta-III Tubulin, which are absent in undifferentiated cells. Bars 20 μm