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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: FLIM imaging revealed spontaneous osteogenic differentiation of stem cells on gradient pore size tissue-engineered constructs

Fig. 3

a Fluorescent images of MSCs on scaffolds stained with calcein and propidium iodide, cell nuclei stained with Hoechst; × 400; b analysis of alkaline phosphatase activity in culture media obtained during cultivation in DMEM and in a medium for the induction of osteogenic differentiation; c staining with Alizarin Red S for the detection of calcifications in MSCs on homogeneous and heterogeneous scaffolds by the 28th day of cultivation in DMEM and in osteogenic medium; × 100. *statistically significant differences compared to the corresponding time point for MSCs on homogeneous scaffolds; Mean ± SD, p-value ≤ 0.05

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Stem Cell Research & Therapy

ISSN: 1757-6512