Fig. 7

Scaffold biodegradation analysis using fluorescence stereo ZOOM microscopy. a Macro-images of implanted scaffolds with either a homogeneous or heterogeneous structure, obtained from the surface of the cranial bone of mice in vivo; the dotted lines mark the boundaries of the scaffolds; the red arrow indicate the formation of new blood vessels; b analysis of scaffold biodegradation based on the scaffold autofluorescence signal in the DAPI channel. The values of the area parameter and the integrated density parameters are presented in arbitrary units. *statistically significant differences in MSCs on heterogeneous scaffolds in comparison with MSCs on homogeneous scaffolds at corresponding time points; Mean ± SD, p < 0.05