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Fig. 7 | Stem Cell Research & Therapy

Fig. 7

From: Side population cells derived from hUCMSCs and hPMSCs could inhibit the malignant behaviors of Tn+ colorectal cancer cells from modifying their O-glycosylation status

Fig. 7

The changing of Cosmc and T-synthase protein, T-synthase, and C3GnT activity in CRC cells before and after co-cultured with SP-hUCMSCs, SP-hPMSCs, and HUVECs for 72 h. a The activity of T-synthase of LS174T-Tn−, LS174T-Tn+, HT-29-Tn−, HT-29-Tn+ cells before and after co-cultured with SP-hUCMSCs, SP-hPMSCs, and HUVECs was detected using a fluorescence method. b The C3GnT activity of LS174T-Tn−, LS174T-Tn+, HT-29-Tn−, HT-29-Tn+ cells before and after co-cultured with SP-hUCMSCs, SP-hPMSCs, and HUVECs was detected using a new fluorescence method. c Cosmc and T-synthase promote LS174T-Tn−, LS174T-Tn+, HT-29-Tn−, HT-29-Tn+ cells before and after co-cultured with SP-hUCMSCs, SP-hPMSCs, and HUVECs detected by Western blotting. Full length original blots are include in Additional file 1: Fig. S3. Three repeated Western blot experiments are include in Additional file 2.  Data shown are the mean ± SD of three independent experiments (*P < 0.05, **P < 0.01, and ***P < 0.001)

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Stem Cell Research & Therapy

ISSN: 1757-6512