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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: An optimized force-triggered density gradient sedimentation method for isolation of pelage follicle dermal papilla cells from neonatal mouse skin

Fig. 3

Characterisation of DP-spheres after second FDGS treatment. A The schematic diagram of FDGS 2st procedure. B Snapshots of live-imaging of NFC cultures after 48 h. Loss of three-dimensional follicle structure after 48 h of NFC culture. Single optical sections showing a single NFC at four consecutive time points (0, 12, 24, and 48 h). Arrows indicate DP-spheres. C Freshly isolated DP-spheres, before (left) and after (right) FDGS2nd treatment. D The transverse-section diameter of obtained DFs, NFCs, and DP-spheres. E Phase-contract microscopic images of freshly isolated DP-spheres and after 3 days of culture, respectively. DF cells were used as the control. F Isolated DP-spheres contained Ki67-positive (green) dividing cells after 2 days of culture. Scale bars: 50 μm. G Quantification of DP and DF cell proliferation rates after three passages in culture. H FACS analysis of Sox2+ cells in dermal cells, NFC and DP cells by flow cytometry.(G) Quantification of the percentage of Sox2+ cells in dermal, NFC and DP cells. (*p < 0.05)

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