Skip to main content
Fig. 9 | Stem Cell Research & Therapy

Fig. 9

From: CINC-2 and miR-199a-5p in EVs secreted by transplanted Thy1+ cells activate hepatocytic progenitor cell growth in rat liver regeneration

Fig. 9

Induction of Il17b expression in SECs by cytokines in Thy1-MC EVs. Administration of CINC-2 and MCP-1 to SECs and Kupffer cells (A). EVs were prepared from the conditioned medium of Thy1-MCs cultured for 2 days and were administered to SECs and Kupffer cells isolated from a normal rat liver. Thy1-EVs were administered 3 h after plating, and the cells were cultured for 48 h. Using a CINC-2 neutralizing antibody or CXCR2 inhibitor (SB225001), the effects of CINC-2/CXCR2 signaling were assessed for the induction of Il17b expression in SECs by CINC-2 and Thy1-EVs, respectively. Il17b expression was measured via quantitative reverse transcription polymerase chain reaction (qRT–PCR) (B). SHs were cultured in serum-free media on Matrigel-coated dishes. SEC-CM with and without CINC-2 treatment were administered to SHs for 3 h after plating and cultured for 7 days. Il17rb expression was measured via qRT–PCR (C). Asterisk indicates significant differences at p < 0.05. EVs extracellular vesicles, MHs mature hepatocytes, SECs sinusoidal endothelial cells, SHs small hepatocytes. Panel C contains the photos of typical SH colonies treated with each cytokine. BrdU was added to the culture medium 24 h before fixation, and immunocytochemistry for BrdU was performed 7 days after plating. Scale bar, 100 μm. Total number of SH colonies per well (D), number of cells per colony (E), and percentage of cells with BrdU+ nucleus per colony (F). Asterisk indicates significant differences at p < 0.05

Back to article page