Fig. 7

Results of proteomic analysis of differentially expressed proteins. Bubble charts of A Biological process; B Cellular component; C Molecular function. Red represents high expression, while blue represents low expression. Significant enrichment (P < 0.05) of differentially expressed proteins was revealed by Fisher's exact test and the top 20 most significantly enriched categories are shown in the bubble chart. The vertical axis of the bubble chart is the functional classification or pathway, and the horizontal axis is the Log2 converted value of the proportion of the differentially expressed protein in the functional type divided by the ratio of the identified proteins. The circle color indicates the enrichment P value, and the circle size indicates the number of differentially expressed proteins in the functional classes or pathways. D Clustering analysis was performed to display the significant differentially expressed proteins in spheroid cells and parent cells of Ishikawa and HEC1A. Red represents higher expression, while blue represents lower expression (Ishikawa: parent 1–3 and spheroid 1–3, HEC1A: parent 4–6 and spheroid 4–6). E KEGG pathway enrichment in spheroid cells and parent cells of Ishikawa and HEC1A. F STRING analysis revealed the protein–protein interaction (PPI) network of the 80 dysregulated proteins. Circles represent differentially expressed proteins, and different colors represent differentially expressed proteins (blue is down-regulated protein, red is up-regulated protein). The circle size represents the number of proteins that are different from each other. The larger the circle, the more proteins it interacts with, the more important the protein is in the network