Fig. 2From: Epithelium-derived SCUBE3 promotes polarized odontoblastic differentiation of dental mesenchymal stem cells and pulp regenerationCell proliferation and migration abilities of hDPSCs treated with exogenous rhSCUBE3. A The expression of TGFβ pathway downstream effectors, TGFβ1, TGFβR1, and TGFβR2, was assessed using RT-qPCR. B The expression of TGFβ pathway downstream effectors was assessed using Western blot analysis. Full-length blots/gels are presented in Additional file 3. c CCK-8 assay. D, E EdU assay and quantification. F, G Wound healing assay and quantification. H, I Transwell migration assay and quantification. n = 3 independent biological samples. *P < 0.05, **P < 0.01, ***P < 0.001. SB, SB431542. hDPSC, human dental pulp stem cellBack to article page