Fig. 5

Histone β-hydroxybutyrylation activates transcription of Gata2. A Screening and validation of differentially expressed genes by Chip-seq. B Distribution of H2BK5bhb sites relative to the transcription start site (TSS). C IGV tracks for Gata2 from Chip-seq data. Con, C: control, Met, T: metformin. D Verification of Gata2 expression by qRT-PCR. (E) Verification of Gata2 expression by western blotting. Uncropped full-length gel blots can be found in Additional file 5: Fig. S5C. F Analysis of FGSCs with Gata2 interference by CCK-8 assays (Scale bars: 20 μm). G EdU assay of FGSCs with Gata2 interference. H EdU assay of Gata2-knockdown FGSCs with metformin treatment. All data are presented as means ± SD of three biological replicates. *p < 0.05, **p < 0.01 compared with the control by two-tailed Student’s t-test. Con: control, Met: metformin