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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: HSPB7 oppositely regulates human mesenchymal stromal cell-derived osteogenesis and adipogenesis

Fig. 4

HSPB7 silencing in BMSCs enhances adipogenesis. A–D Representative images (A) and quantitative data (B) of Oil Red O staining performed after 14 days with adipogenic induction. Total cell number was determined by DAPI staining (C). Quantitative data of Oil Red O staining were adjusted by cell number (D). E The expression of adipogenic markers was evaluated by qRT-PCR at indicated time points. F–G Representative images (F) and quantitative data (G) of adipogenic genes were assessed on day 7 following adipogenic induction by Western blot. Full-length blots are presented in Additional file 7: Fig. S6. Abbreviations: Peroxisome proliferator-activated receptor gamma (PPARγ), Fatty acid binding protein 4 (FABP4), CCAAT/enhancer binding protein alpha (C/EBPα) and Perilipin-1 (PLIN1) Fatty acid synthase (FASN) and Acetyl-CoA carboxylase (ACC). Data are presented as means ± SEM and analyzed by one-way ANOVA (B–D and G, n = 3–4 per group) or two-way ANOVA (E, n = 4 per group) followed by post-hoc testing. Scale bars: 200 μm

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