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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: HSPB7 oppositely regulates human mesenchymal stromal cell-derived osteogenesis and adipogenesis

Fig. 6

The ability of HSPB7 to enhance osteogenic differentiation depends on N- and C-terminal domains. A A schematic representation of full-length HSPB7 and its truncated mutants. His tag is located at the C terminal in each construct B 3D structures of FL HSPB7 and its truncated mutants generated by SWISS-MODEL (https://swissmodel.expasy.org). These models were predicated with reference to the template of 4jut.1.A and were selected on the basis of GMQE and QMEANDisCo global scores. C BMSCs expressing the indicated HSPB7 constructs were immunoblotted with anti-histidine antibody in non-differentiating conditions. Control samples were transduced with dsRED. Full-length blots are presented in Additional file 7: Fig. S6. D ALP activity was evaluated in BMSCs expressing dedicated constructs under osteogenic induction at day 13. E, F Osteoblast mineralization was assessed by calcium deposition assay (E) and Alizarin Red S staining (E) after three weeks osteogenic induction in BMSCs. Abbreviations: ΔN, N-terminus deletion; ΔC, C-terminus deletion; SRS, serine-rich sequence stretch; FL, full-length. Data are presented as means ± SEM and analyzed by one-way ANOVA (n = 4 per group) followed by post-hoc testing

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