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Fig. 7 | Stem Cell Research & Therapy

Fig. 7

From: HSPB7 oppositely regulates human mesenchymal stromal cell-derived osteogenesis and adipogenesis

Fig. 7

Blocking Activin A overcomes HSPB7 silencing-mediated osteogenic inhibition. A INHBA mRNA expression was assessed by qRT-PCR following HSPB7 silencing at different time points following osteogenic induction. B, C INHBA mRNA expression was assessed by Western blot following HSPB7 silencing at day 14 (B) and day 21 (C) following osteogenic induction. Full-length blots are presented in Additional file 7: Fig. S6. D Osteoblast mineralization was assessed by calcium deposition assay after three weeks osteogenic induction. BMSCs were induced to osteogenic differentiation following HSPB7 silencing in the presence of SB431542 (D) or neutralizing Activin A antibodies (E). F ECM genes were evaluated by qRT-PCR following HSPB7 silencing at day 13 and day 20. Data are presented as means ± SEM and analyzed by two-tailed Student’s t-test (B–C, n = 3 per group) or two-way ANOVA (A, D–F, n = 4 per group) followed by post-hoc testing

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