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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Maternal dendritic cells influence fetal allograft response following murine in-utero hematopoietic stem cell transplantation

Fig. 1

Transient suppression of maternal dendritic cells with diphtheria toxin resulted in reduced maternal immune cell trafficking to fetal recipients following intrauterine transplantation of semi-allogenic and fully allogenic donor cells, and effect on donor cell chimerism. Selective reduction of conventional dendritic cells (cDC) and subtypes (cDC1, cDC2) in various organs of non-pregnant CD11c.DTR females (n = 5) after administering diphtheria toxin (DT, arrows, a). Schematic diagram representing intrauterine transplantations of maternal (mIUT, n = 31) paternal (pIUT, n = 60) and fully allogenic (aIUT, n = 22) cells performed at E14 (b), with or without DC-depletion to the dam. Maternal immune cell microchimerism (MMc) levels were similar in DC-depleted and DC control group with each IUT (c), higher in aIUT versus pIUT, and higher in the DC-depleted bone marrow (BM) versus peripheral blood (PB) after mIUT (black*). Donor cell chimerism (DCC) in the DC control group was highest in BM and PB (black*) of pIUT, and higher in mIUT PB vs BM (red*). With the DC-depleted group, DCC was maintained in pIUT BM (black*) and was higher in mIUT BM versus PB (red*). Maternal cDC, CD4, CD19 cells were non-significantly reduced in the DC controls of recipient BM and higher in PB (d). cDC and subtypes were only found in both groups of the aIUT recipients. Data represent mean ± SD, analysed by two-way ANOVA with Tukey’s multiple comparisons test

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