Fig. 4

Immune profiles of engrafted donor, trafficked maternal and recipient cells following allogenic (C3H) donor cell transplantation and immune T cell responses, cytokine and FOXP3 gene expression following re-exposure to donor cells. Donor microchimerism < 1% was observed in BM and PB (a, b). No differences in donor, maternal (c, d) or recipient (e, f) immune cell profiles were observed in DC-depleted and DC control groups. Within the DC-depleted group, CD4+ central memory (Tm), CD4+ and CD8+ effector (Teff) and regulatory T cells CD25+ Treg and CD62L+ CD25+ Treg were elevated above the DC control group and uninjected controls on exposure to C3H, B6 or BALB/c donor cells (g, h). Cytokine levels from DC-depleted aIUT recipients were significantly higher compared to DC control pups when stimulated with C3H, B6 or BALB/c donor cells, except for IL-1b and FOXP3 (i). The horizontal dotted line represents levels in the uninjected control group normalised to a value of 1 (g–i). Data represent mean ± SD, analysed by two-way ANOVA with Tukey’s multiple comparisons test