Fig. 5

MSCs and IronQ combined treatment decreased the inflammatory response in mice with ICH. A, B The results of double immunofluorescence displayed co-localization for F4/80 co-expressing with IL-6 and TNF-α in the ipsilateral striatum of the mice brain tissue section of each group. Respective change in color showing co-localized signals in merged figures corresponds to Red + Green = Yellow; Red + Blue = Magenta. Scale bar = 100 μm (top panel). The white box areas are shown at higher magnification in the bottom panel. C-D showed the statistical graph of positive IL-6 and TNF-α staining cells (n = 5 for each group; ***P < 0.001, and ****P < 0.0001). E–G The results and analyses of western blot of IL-6 and TNF-α protein expression in each group (n = 5 for each group; *P < 0.05, and ****P < 0.0001)