Fig. 8

The results and analyses of qPCR and western blot revealed that the conditioned medium of MSCs with IronQ reduced inflammatory response by regulating the Mincle/syk signaling pathway. A-C Presentation of graphs of A Mincle, B IL-6, and C TNF-α mRNA expression levels in different cell groups after 6 h (n = 3 for each group; *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001). D-J The results and analyses of western blot demonstrated the protein expression levels of IL-6, TNF-α, Mincle, p-syk, and p-NFκB-p65 in each cell group. D, G Representative immunoblot showing the effect of conditional medium on protein expression of IL-6, TNF-α, Mincle, syk, p-syk, NFκB-p65, p-NFκB-p65, and GAPDH (E–F, H–J); the quantitative densitometric ratio of E IL-6, F TNF-α, H Mincle relative to GAPDH, I p-syk relative to syk, J p-NFκB-p65 relative to NFκB-p65 (n = 3 for each group; ****P < 0.0001). Conditional medium of MSCs with IronQ reduced Mincle and its downstream p-syk, and p-NFκB-p65 protein expression significantly compared with other treatment groups after 24 h