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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Obesity and dyslipidemia are associated with partially reversible modifications to DNA hydroxymethylation of apoptosis- and senescence-related genes in swine adipose-derived mesenchymal stem/stromal cells

Fig. 3

Obesity/dyslipidemia-induced 5hmC marks correlate with changes in gene expression in pathways related to apoptotic process, cell proliferation, and leukocyte-mediated cytotoxicity. A Scatterplot of gene sets from the Molecular Signatures Database identifying those with concordant dysregulation of 5hmC levels and mRNA expression in swine Obese- versus Lean-MSCs. For each gene set, the normalized enrichment score (NES) is separately calculated for hMeDIP-seq (vertical axis) and mRNA-seq (horizontal axis) using Gene Set Enrichment Analysis. Based on thresholds (dashed lines) of NES > 1.4 and NES < -1.4, gene sets with higher (n = 95) or lower (n = 7) levels of both 5hmC and mRNA in Obese- versus Lean-MSCs were marked as red or blue dots, respectively. Genes from the gene sets with B NES > 1.4 or C NES < -1.4 for both hMeDIP-seq and mRNA-seq were extracted, and classified via Gene Ontology (GO): Biological Process overlap analysis. In panels (B, C), salient overlapping gene categories are highlighted in yellow

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Stem Cell Research & Therapy

ISSN: 1757-6512