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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Obesity and dyslipidemia are associated with partially reversible modifications to DNA hydroxymethylation of apoptosis- and senescence-related genes in swine adipose-derived mesenchymal stem/stromal cells

Fig. 6

Functional assays indicate increased senescence in swine Obese-MSCs. A Cell proliferation, given as change in % confluence across 24 h (mean ± SEM; Obese-MSCs: n = 6, Lean-MSCs: n = 5) was not significantly different between Obese- and Lean-MSCs. B Obese-MSCs, but not Lean-MSCs, showed resistance to induction of apoptosis by staurosporine (dissolved in DMSO; 20 nM for 24 h in vitro; DMSO: n = 5 each; staurosporine: n = 6 each). C Percentage of senescence-associated beta-galactosidase (SA-β-Gal) positively stained cells was increased in Obese- versus Lean-MSCs (n = 4 per group). D Obese-MSCs also showed increased p16 immunoreactivity (% staining area), compared with Lean-MSCs (n = 6 per group). *p-value ≤ 0.05 versus Lean-MSCs; #p-value ≤ 0.05 versus Lean-MSCs + DMSO; ‡p-value ≤ 0.05 versus Obese-MSCs + DMSO

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Stem Cell Research & Therapy

ISSN: 1757-6512