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Fig. 7 | Stem Cell Research & Therapy

Fig. 7

From: Obesity and dyslipidemia are associated with partially reversible modifications to DNA hydroxymethylation of apoptosis- and senescence-related genes in swine adipose-derived mesenchymal stem/stromal cells

Fig. 7

Epigenetic reprogramming of swine MSCs with vitamin-C partly reverses obesity/dyslipidemia-induced changes in hydroxymethylation of genes related to apoptosis and cell proliferation/senescence. A Scatterplot of genes in which vitamin-C induced differential 5hmC levels in Obese-MSCs. Genes showing differential 5hmC levels both in Obese- versus Lean-MSCs (x-axis) and in vitamin-C-treated Obese-MSCs versus untreated Obese-MSCs (y-axis) are colored. Differential 5hmC levels entail p-value ≤ 0.05 and fold change ≥ 1.4 (high 5hmC levels) or ≤ 0.7 (low 5hmC levels). Outliers (with very large fold change values) are represented with triangle markers. Green points (n = 40) represent genes with low 5hmC levels in Obese- versus Lean-MSCs, which are reversed upon vitamin-C treatment, while orange points (n = 28) represent genes with high 5hmC levels in Obese- versus Lean-MSCs, which are reversed upon vitamin-C treatment. B Heat map of genes for which vitamin-C treatment reverses the differential 5hmC levels observed in Obese-MSCs (orange and green clusters from panel A), filtered for apoptotic process (GO:0006915), cell cycle (GO:0007049), cell population proliferation (GO:0008283), and cellular senescence [REACTOME SuperPath + (GO:0090398)]. n = 3 per group

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Stem Cell Research & Therapy

ISSN: 1757-6512