Figure 4
Paraffin wax 5 μm-section pellets were stained with safranin-O/fast green stain. All photomicrographs represent low (4x objectives) images. (A-D) 34-year-old woman (BM68), (F-I) 51-year-old man (BM73), (K-N) 43-year-old man (BM74) and (P-S) 62-year-old man (BM84). Scale bar is 100 μm. Biochemical assay of pellets for sulfated glycosaminoglycan (GAG) per DNA content. Pellets were cultured for three weeks in a defined serum-free chondrogenic medium under low (3%) or normal (21% O2) oxygen tension. Data are mean ± standard error of triplicates. (E) 34-year old woman (BM68), (J) 51-year-old man (BM73), (O) 43-year-old man (BM74) and (T) 62-year-old man (BM84). Data represent mean ± standard error of triplicate pellets; one-way ANOVA with Tukey's post hoc test: n.s. (not significant), * = P < 0.05, ** = P < 0.001 and *** = P < 0.0001. ANOVA, analysis of variance.