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Figure 3 | Stem Cell Research & Therapy

Figure 3

From: Production and validation of a good manufacturing practice grade human fibroblast line for supporting human embryonic stem cell derivation and culture

Figure 3

NclFed1A supports human embryonic stem cell (hESC) derivation and culture. (a) The proportion of outgrowths and hESC lines produced by using NclFed1A and MEFS (derived from CF1 and C57Bl6 strains) as feeder cells. (b) hESCs cultured on NclFed1A for 24 passages were positive for the pluripotency markers NANOG and OCT4 (scale bar, 100 μm). (c) A greater proportion of cells expressed Tra-1-60 when cultured on NclFed1A than on MRC-5 (P < 0.05); no significant difference was noted between the expression of pluripotency markers after culture on NclFed1A, iHDFn, and iMEF. (d) The expression of pluripotency markers was similar after culture on NclFed1A at PD8, PD18, and PD28; at PD38, fewer cells expressed Tra-1-81 and SSEA4 (P < 0.05). (e) hESCs cultured on NclFed1A at P15 had normal hESC morphology. (f) hESCs cultured on NclFed1A at PD38 showed signs of differentiation (arrows).

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Stem Cell Research & Therapy

ISSN: 1757-6512