Table 3 The characteristics of NclFed1A
Characteristics | Result |
|---|---|
Characterization of NclFed1A | Â |
Expression of fibroblast markers | Expresses HFFCellect, 5B5, CD90, CD44, and CD160 |
Karyology | Normal male karyotype |
Identification | STR analysis determined |
Cell-doubling time | 26.7 ± 13 hours |
Human viruses HIV 1 and 2 provirus, HTLV 1 and 2 provirus, HAV, HBV, HCV, HHV-6, HHV-7, HHV-8, hCMV, EBV, SV40, and B19 | Not detected |
Bovine viruses (9CFR) | Not detected |
Ability to support hESCs | Â |
Ability to support hESC derivation | 33% (three of nine blastocysts plated) |
Ability to support hESC culture | Yes (up to 28 population doublings from the MCB) |