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Table 3 The characteristics of NclFed1A

From: Production and validation of a good manufacturing practice grade human fibroblast line for supporting human embryonic stem cell derivation and culture

Characteristics Result
Characterization of NclFed1A  
Expression of fibroblast markers Expresses HFFCellect, 5B5, CD90, CD44, and CD160
Karyology Normal male karyotype
Identification STR analysis determined
Cell-doubling time 26.7 ± 13 hours
Human viruses HIV 1 and 2 provirus, HTLV 1 and 2 provirus, HAV, HBV, HCV, HHV-6, HHV-7, HHV-8, hCMV, EBV, SV40, and B19 Not detected
Bovine viruses (9CFR) Not detected
Ability to support hESCs  
Ability to support hESC derivation 33% (three of nine blastocysts plated)
Ability to support hESC culture Yes (up to 28 population doublings from the MCB)