Figure 6
From: Orthopaedic applications of nanoparticle-based stem cell therapies
Cell lines can be guided towards different mesenchymal lineages for orthopaedic applications. Histological and immunohistochemistry staining of human bone marrow mesenchymal stem cells (HBMSC); HBMSC + transforming growth factor (TGF) β3, HBMSC labelled with TREK-1, or HBMSC labelled with RGD particles encapsulated in alginate/chitosan capsules and exposed to a pulsed magnetic field for 21 days in vitro. Tissue sections (6 μm) were stained for sulphated mucins using Alcian blue and for collagen using (A to D) Sirius red, (E to H) collagen type 1 and (I to L) collagen type 2. Scale bars = 100 μm.